- Administration of the same dose of drugs with different Vd will result in lower initial conc and slower rate of elimination in the drugs with larger Vd. (if CL_T is constant). Slower drug elimination results in slower rate of metabolite formation.

- Administration of the same dose of drugs that have different Vd lead to the formation of the same amount of the metabolite if the fraction of the drug converted to the metabolite (f_m) is constant.

- Since the metabolite parameters are similar and the same amount of metabolite is formed in vivo, the metabolite AUC will be similar regardless of the parent drug Vd and CL_T.

The volume of distribution is the apparent volume in which the drug can be distributed in. It is the factor that relates the amount of drug in the body to the concentration of the drug in the sampling site. It is not an actual volume, however it is a hypothetical volume determined by the drug distribution behavior.

The volume of distribution ranges from 3-5 liters to more than 25 L/kg. Drugs with higher affinity for tissues, have higher volume of distribution.